※一部利用できない機能があります
| 1.
論文 |
論文
|
Hara, Akinori ; Furuichi, Kengo ; Koshino, Akihiko ; Yasuda, Haruka ; Tran, Trang Thi Thu ; Iwata, Yasunori ; Sakai, Norihiko ; Shimizu, Miho ; Kaneko, Shuichi ; Nakamura, Hiroyuki ; Wada, Takashi ; 原, 章規 ; 古市, 賢吾 ; 岩田, 恭宜 ; 坂井, 宣彦 ; 清水, 美保 ; 金子, 周一 ; 中村, 裕之 ; 和田, 隆志
概要:
金沢大学医薬保健研究域医学系<br />Introduction: We examined the impact of autoantibodies on the erythropoietin receptor (EPOR) in type
…
2 diabetic patients with chronic kidney disease (CKD). Methods: A total of 112 Japanese patients with type 2 diabetes who had CKD were enrolled in this study and followed for a mean of 45 months. Sera from these patients were screened for anti-EPOR antibodies using enzyme-linked immunosorbent assays. Results: Anti-EPOR antibodies were detected in 26 patients (23%). Anti-EPOR antibodies were associated with low hemoglobin concentrations and decreased renal function. In patients with biopsy-proven diabetic nephropathy, anti-EPOR antibodies were associated with increased levels of interstitial inflammation. A decrease in renal function was observed more frequently in patients with antibodies than in those without antibodies, and the presence of the antibodies together with well-known clinical parameters, including proteinuria and low glomerular filtration rate, was a significant risk factor for end-stage renal disease. In human tubular epithelial HK-2 cells, IgG fractions containing anti-EPOR antibodies upregulated the expression of monocyte chemoattractant protein-1 mRNA under a high concentration of glucose. Conclusion: Anti-EPOR antibodies might be involved in the progression of renal lesions and in the impaired erythropoiesis in type 2 diabetic patients with CKD. Furthermore, the presence of anti-EPOR antibodies may be an additional predictor for end-stage renal disease in type 2 diabetes. © 2017 International Society of Nephrology<br />Embargo Period 12 months
続きを見る
|
||||
| 2.
論文 |
論文
|
Konno, Tetsuo ; Hayashi, Kenshi ; Fujino, Noboru ; Nagata, Yoji ; Hodatsu, Akihiko ; Masuta, Eiichi ; Sakata, Kenji ; Nakamura, Hiroyuki ; Kawashiri, Masa-aki ; Yamagishi, Masakazu ; 今野, 哲雄 ; 林, 研至 ; 藤野, 陽 ; 永田, 庸二 ; 寳達, 明彦 ; 坂田, 憲治 ; 中村, 裕之 ; 川尻, 剛照 ; 山岸, 正和
概要:
金沢大学医薬保健研究域医学系<br />Background: Myocardial scarring can be assessed by cardiac magnetic resonance imaging with late gado
…
linium enhancement and by endomyocardial biopsy. However, accuracy of late gadolinium enhancement for predicting microscopic myocardial scarring in biopsied specimens remains unknown in hypertrophic cardiomyopathy. We investigated whether late gadolinium enhancement in the whole heart reflects microscopic myocardial scarring in the small biopsied specimens in hypertrophic cardiomyopathy. Methods and Results: Twenty-one consecutive patients with hypertrophic cardiomyopathy who were examined both by cardiac magnetic resonance imaging and by endomyocardial biopsy were retrospectively studied. The right interventricular septum was the target site for endomyocardial biopsy in all patients. Late gadolinium enhancement in the ventricular septum had an excellent sensitivity (100%) with a low specificity (40%) for predicting microscopic myocardial scarring in biopsied specimens. The sensitivity of late gadolinium enhancement in the whole heart remained 100% with a specificity of 27% for predicting microscopic myocardial scarring in biopsied specimens. Quantitative assessments of fibrosis revealed that the extent of late gadolinium enhancement in the whole heart was the only independent variable related to the microscopic collagen fraction in biopsied specimens (β = 0.59, 95% confident interval: 0.15-1.0, p = 0.012). Conclusions: Although there was a compromise in the specificity, the sensitivity of late gadolinium enhancement was excellent for prediction of microscopic myocardial scarring in hypertrophic cardiomyopathy. Moreover, the severity of late gadolinium enhancement was independently associated with the quantitative collagen fraction in biopsied specimens in hypertrophic cardiomyopathy. These findings indicate that late gadolinium enhancement can reflect both the presence and the extent of microscopic myocardial scarring in the small biopsied specimens in hypertrophic cardiomyopathy. © 2014 Konno et al.
続きを見る
|
||||
| 3.
論文 |
論文
|
Higashi, Tomomi ; Ohkura, Noriyuki ; Fujimura, Masaki ; Nakai, Satoshi ; Honda, Yasushi ; Saijoh, Kiyofumi ; Hayakawa, Kazuichi ; Kobayashi, Fumihisa ; Michigami, Yoshimasa ; Olando, Anyenda Enoch ; Hitomi, Yoshiaki ; Nakamura, Hiroyuki ; 東, 朋美 ; 大倉, 徳幸 ; 藤村, 政樹 ; 西條, 淸史 ; 早川, 和一 ; 小林, 史彦 ; 道上, 義正 ; 人見, 嘉哲 ; 中村, 裕之
概要:
金沢大学医薬保健研究域医学系<br />Asian dust, known as kosa in Japanese, is a major public health concern. In this panel study, we eva
…
luated the effects of exposure to kosa on daily cough occurrence. The study subjects were 86 patients being treated for asthma, cough variant asthma, or atopic cough in Kanazawa University Hospital from January 2011 to June 2011. Daily mean concentrations of kosa and spherical particles were obtained from light detection and ranging (LIDAR) measurements, and were categorized from Grade 1 (0 μg/m3) to 5 (over 100 μg/m3). The association between kosa and cough was analyzed by logistic regression with a generalized estimating equation. Kosa effects on cough were seen for all Grades with potential time lag effect. Particularly at Lag 0 (the day of exposure), a dose-response relationship was observed: the odds ratios for Grades 2, 3, 4, and 5 above the referent (Grade 1) were 1.111 (95% confidence interval (CI): 0.995-1.239), 1.171 (95% CI: 1.006-1.363), 1.357 (95% CI: 1.029-1.788), and 1.414 (95% CI: 0.983-2.036), respectively. Among the patients without asthma, the association was higher: the odds ratios for Grades 2, 3, 4 and 5 were 1.223 (95% CI: 0.999-1.497), 1.309 (95% CI: 0.987-1.737), 1.738 (95% CI: 1.029-2.935) and 2.403 (95% CI: 1.158-4.985), respectively. These associations remained after adjusting for the concentration of spherical particles or particulate matter with an aerodynamic diameter of less than 2.5 μm (PM2.5). Our findings demonstrate that kosa is an environmental factor which induces cough in a dose-response relationship. © 2014 Elsevier B.V.<br />Embargo Period 12 months
続きを見る
|
||||
| 4.
その他 |
その他
|
金沢大学フロンティアサイエンス機構 ; 長谷川, 浩 ; 桑原, 貴之 ; 児玉, 昭雄 ; 徳田, 規夫 ; 田中, 康規 ; 中村, 裕之 ; 井上, 啓 ; 堀, 修 ; 平尾, 敦 ; 横井, 毅 ; 土屋, 弘行 ; 宮地, 利明 ; 多久和, 陽 ; 白土, 明子 ; Hasegawa, Hiroshi ; Kuwabara, Takayuki ; Kodama, Akio ; Tokuda, Norio ; Tanaka, Yasunori ; Nakamura, Hiroyuki ; Inoue, Hiroshi ; Hori, Osamu ; Hirao, Atsushi ; Yokoi, Tsuyoshi ; Tsuchiya, Hiroyuki ; Miyachi, Toshiaki ; Takuwa, Yoh ; Shiratsuchi, Akiko
|
||||
| 5.
論文 |
論文
|
中村, 裕之 ; Nakamura, Hiroyuki
概要:
金沢大学医薬保健研究域医学系<br />アレルギー疾患、特に難治性の気管支喘息と肥満の合併が、共通した食生活の問題から生じることが指摘されている。25年度にリクルートできた年齢3-5歳の児で3年間に喘息が消失した人の子4人と、存続した子5人
…
、花粉症が存続した4人について、採取した糞便より、腸内菌種解析とメタゲノム解析を行った。その結果、3群間には、炭水化物摂取の差と、Enterobacteriaceaeなどの発現パターンの差が認められた。特に、喘息消失群と存続群ではBidfidobacteriuの発現パターンと体重増加の関係が認められることから、小児の喘息の消失には、炭水化物などの摂取を控えた体重増加の抑制が有効であると推測された。<br />The combination of allergic diseases such as asthma and obesity or overweight are considered to be produced by the eating habits. We have performed 3 years follow-up study on infants aged 3-5 years from 2013 and analyzed the strain of intestinal flora and metagenome. Four infants disappeared asthma and 5 did not, and 4 still showed pollinosis. We have observed the differences in the pattern of the expressions of enterobacteriaceae together with the intake of carbohydrates among the three groups. There was a correlation between the pattern of the expressions of the bidfidobacteriu and the increase in weight of infants after comparing between the infants with and without the disappearance of asthma. These results suggest that inhibiting unnecessary intake of carbohydrates, subsequently suppressing the excess of weight gain lead to the disappearance of asthma in infants.<br />研究課題/領域番号:25670295, 研究期間(年度):2013-04-01 - 2016-03-31
続きを見る
|
||||
| 6.
論文 |
論文
|
中村, 裕之 ; Nakamura, Hiroyuki
概要:
金沢大学医薬保健研究域医学系<br />アレルギー性疾患の原因については、胎児期あるいは小児期の前半に最も頻繁に感染するRespiratory syncytialウイルスなどのウイルスの関与がよく知られるようになったが、その病態の背景である
…
感受性遺伝子との相互作用はよく知られていない。妊娠モデルマウスの児の脾臓細胞のIL4RA遺伝子におけるCpG部位のメチル化と感染の関係が出産直後において認められた。疫学研究では、臍帯血単核球のIL4RAにおけるCpG部位のメチル化がハウスダスト感作による喘息との関係において認められた。これらの結果から、出生直後の児において感染が成立するとともにアレルギー関連の遺伝子が発現していると考えられた。<br />The infection of virus such as respiratory syncytial virus is involved in pathogenesis of allergy during prenatal and postnatal infants, although the interaction between candidate genes and virus infection remains to be elucidated. We demonstrated hypomethylation of CpG islands produced by virus infection in IL4RA gene in splenic cells for postpartum infants from pregnant model mouse. Epidemiologic study showed the hypomethylation of CpG islands in in IL4RA gene in monocytes in cord blood of pregnant patients with asthma sensitized by house dust. These results suggest expression of IL4RA gene in just new born infants is produced by infection of RS virus.<br />研究課題/領域番号:23390160, 研究期間(年度):2011-04-01 - 2014-03-31
続きを見る
|
||||
| 7.
論文 |
論文
|
中村, 裕之 ; Nakamura, Hiroyuki
概要:
金沢大学医薬保健研究域医学系<br />リポソームカプセルのリン脂質2重層にNKT細胞活性化物質「α-GalCer」を挿入し、スギ花粉T細胞エピトープを封入し、CTLエピトープを表面に結合することによってリポソームワクチンを構築した。インフ
…
ルエンザウイルス感染およびスギ花粉症モデルマウスを対象に、リポソームワクチンを6日間、予防的に投与し、IFV抗体価を指標として検討した結果、新しいインフルエンザウイルスワクチンの有効性が証明された。<br />We constructed liposomes vaccines, in α-GalCe, an activated substance in NKT cell was inserted into the bilayers of phospholipid liposome capsules, and T cell epitopes derived from Japanese cedar pollen was included and attached to the surface of CTL epitopes. We examined IFV titers in the model mouse infected by influenza virus and sensitized by Japanese cedar pollen. In results, the administration of the vaccines for 6 days before the infection showed the prophylactic effects.<br />研究課題/領域番号:22659121, 研究期間(年度):2010-2012
続きを見る
|
||||
| 8.
論文 |
論文
|
中村, 裕之 ; Nakamura, Hiroyuki
概要:
アレルギー性疾患の発症におけるRSウイルスによる感染の役割を解明し、分子遺伝マーカーを同定することによって予防法を開発することを目的とした。動物実験と分子疫学によって、その分子遺伝マーカーとして、Th2サイトカイン、LR4とCD14が同定さ
…
れた。RSウイルス由来のS糖タンパク遺伝子を用いたDNAワクチンは、この活性を抑制することによってアレルギー予防につながることが見出され、その有効性が示された。<br />This study aimed to clarify the involvement of RS virus in allergic diseases and develop preventive methods by determining the molecular and genetic marker in it. Animal experiments and molecular epidemiology determined Th2 cytokines, LR4 and CD4 as the markers. DNA vaccine containing S glycoprotein gene was found to lead to the inactivation of the markers, subsequently resulting in the efficient preventive agents.<br />研究課題/領域番号:20390170, 研究期間(年度):2008-2010
続きを見る
|
||||
| 9.
論文 |
論文
|
中村, 裕之 ; Nakamura, Hiroyuki
概要:
アレルギー性疾患における環境と遺伝の相互作用の解明によってオーダーメード予防法を新たに提示するために、気管支喘息症とスギ花粉症患者における好酸球関連蛋白であるCC chemokine receptor(CCR)familyとEotaxin/
…
CCL11,Eotaxin-2/CCL24,Eotaxin-3/CCL26、Monocyte chemoattractant protein-1(MCP-1,CCL2)の遺伝子多型を健常者と比較した。喘息症および花粉症におけるCCR3遺伝子の51C、CCL26の2497G、2563C、MCP-1遺伝子の2518Gの頻度が健常群に対して有意に多かった。また、これらの蛋白と重症度においても相関が認められた。したがってアレルギー発症および重症化におけるこれらの好酸球関連蛋白の臨床的な意義も証明された。また環境化学物質除去を目的として非晶鉄および活性炭を含む除去フィルターを新たに開発し、このフィルターでディーゼル排気粒子(Diesel exhaust particulate,DEP)を含む水をろ過し、アレルギーモデルマウスに投与し、気管支喘息の予防効果および軽減効果を検証した。そのフィルターによるDEP中に含まれるアレルギー促進物質の除去を試み、マウスへ投与を行ったところ、肺への好酸球および好中球の浸潤が有意に抑制され、ダニ抗原特異的IgG1抗体価も有意に下がった。病理標本上からも肺の炎症が抑制されていることがわかった。以上より新たに開発したフィルターが環境化学物質の除去によってアレルギーの発症を予防できることを示すことができた。<br />To propose tailor-made preventive methods for allergic diseases by clarification between environment and genes, we compared single nucleotide polymorphisms (SNP) of genes of eosinophil-related protein, e.g., CC chemokine receptor (CCR) family, Eotaxin/CCL11, Eotaxin-2/CCL24, Eotaxin-3/CCL26, Monocyte chemoattractant protein -1 (MCP-1, CCL2) between patients with asthma or Japanese cedar pollinosis and controls. We recognized significantly higher frequencies of 51 C in CCR3 gene, 2497G and 2563C in CCL26 gene, and 2518G in MCP-1 gene in patients with asthma or Japanese cedar pollinosis as compared to controls. There were significant correlations between serum levels of those proteins and severity of those allergic diseases. These results suggest the clinical significance of those eosinophils-related proteins in allergic diseases. Next, we developed the novel preventive methods for allergy, using the filter system incorporating activated carbon and amorphous iron(hydr)oxide, which efficiently removes diesel exhaust particulate (DEP). We demonstrated that removal of DEP by the filter system produced significant inhibition of allergic responses, e. g, eosinophils and neutrophils infiltration into lung tissues, lung inflammation and elevated Der f specific IgGl antibody level in asthma model mice BALB/c, which were sensitized and stimulated with Der f. Therefore, this filter system might be the most effective to prevent allergy development, if its use were started in our life as early as possible (e.g. in our infancy).<br />研究課題/領域番号:17390174, 研究期間(年度):2005-2007<br />出典：「環境中物質と好酸球関連蛋白遺伝子の相互作用解明によるアレルギー疾患の予防」研究成果報告書 課題番号17390174 (KAKEN：科学研究費助成事業データベース（国立情報学研究所）) 本文データは著者版報告書より作成
続きを見る
|
||||
| 10.
論文 |
論文
|
中村, 裕之 ; Nakamura, Hiroyuki
概要:
本研究では、マイクロ波によって生じる子宮胎盤循環不全と胎盤機能障害に対して胎児側の神経内分泌系を中心とした防御機構の役割を明らかにする。マイクロ波暴露装置を用いて、妊娠ラットにAmerican Conference of Industria
…
l Hygienists(ACGIH)とAmerican National Institute(ANSI)の環境基準である0.4W/kgのマイクロ波を暴露し、暴露中の胎児側胎盤血流量及び子宮側胎盤血流量をモニターした。胎児試料から視床下部と下垂体の脳組織試料を作成し、胎児視床下部CRHと、胎児下垂体オピオイド受容体を評価した。その結果、CRH受容体拮抗剤であるα-helical CRH(9-41)の前投与によっては、子宮胎盤血流量の低下は起こらないことがわかった。また、cyclooxygenase拮抗剤であるindomethacinの前投与によっても、血流量の低下を抑制できた。マイクロ波暴露に際し、胎児視床下部CRHのmRNAの発現は認められなかったが、α-helical CRH(9-41)あるいはindomethacinの投与によって認められたことから、0.4W/kgのマイクロ波暴露では、胎児視床下部CRHが母胎の胎盤循環機能の制御にすでに関わることで、胎児の防御機構が働くことが示唆された。一方、環境基準の5倍の2.0W/kgを暴露させた場合、その防御機構は働かず、母胎ともに温度上昇がもたらすことが認められた。したがって、現行の安全基準内であれば、防御機構は働き、十分、そのマイクロ波の暴露は、安全であることが示された。環境基準を大きく超えて、温度上昇をもたらすマイクロ波暴露は、妊娠の経過に、温度上昇による諸影響を与える可能性は示唆された。<br />We tried to clarify preventing mechanisms including neuroendocrine systems of fetus for uteroplacental circulatory disturbances and uteroplacental dysfunctions produced by microwaves. We simulated the microwave condition in pregnant rats for the specific absorption ratio (SAR) of 0.4 W/kg, which is the maximum permissible exposure level recommended by the American Conference of Industrial Hygienists (ACGIH) and the American National Institute (ANSI). The pretreatment of intravenous (iv) administration of CRH receptor antagonist, α-helical CRH (9-41) as well as a cyclooxygenase inhibitor indomethacin prevented the uteroplacental circulatory disturbances during microwaves. Although microwaves did not induce fetal hypothalamic CRH mRNA change, the administration of bosentan or indomethacin before microwave exposure did it These results suggest a preventing system of uteroplacenta-fetus against microwaves in which fetal hypothalarnic CRH is involved in mediating uteroplacental circulation during microwaves at 0.4 W/kg. By contrast, we found that the preventing systems did not work during microwaves at 2.0 W/kg, subsequently resulting a significant increase in colonic temperature and decrease in uteroplacental blood flow. These results suggest microwaves at the SAR of 0.4 W/kg, which is the maximum permissible exposure level, is within safety levels without producing uteroplacental dysfunctions. Microwaves much above the maximum permissible level may show some harmful changes during pregnancy associated with an increase in colonic temperature produced microwaves.<br />研究課題/領域番号:13470079, 研究期間(年度):2001-2002<br />出典：「マイクロ波による子宮胎盤循環及び胎盤機能障害に対する胎児側防御機構の解明」研究成果報告書 課題番号13470079 (KAKEN：科学研究費助成事業データベース（国立情報学研究所）) 本文データは著者版報告書より作成
続きを見る
|
