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Simultaneous Determination of Methamphetamine and Its Metabolites in the Urine Samples of Abusers by High Performance Liquid Chromatography with Chemiluminescence Detection

フォーマット:
論文
責任表示:
Hayakawa, Kazuichi ; Miyoshi, Yasuko ; Kurimoto, Hiroaki ; Matzushima, Yuko ; Takayama, Nariaki ; Tanaka, Seishi ; Miyazaki, Motoichi ; 早川, 和一 ; 三好, 裕子 ; 栗本, 博昭 ; 松島, 優子 ; 高山, 成明 ; 田中, 星司 ; 宮崎, 元一
言語:
英語
出版情報:
Pharmaceutical Society of Japan 日本薬学会, 1993-09-15
著者名:
Hayakawa, Kazuichi
Miyoshi, Yasuko
Kurimoto, Hiroaki
Matzushima, Yuko
Takayama, Nariaki
Tanaka, Seishi
Miyazaki, Motoichi
早川, 和一
三好, 裕子
栗本, 博昭
松島, 優子
高山, 成明
田中, 星司
宮崎, 元一
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掲載情報:
Biological and Pharmaceutical Bulletin
ISSN:
0918-6158  CiNii Research  Webcat Plus  JAIRO
1347-5215  CiNii Research  Webcat Plus  JAIRO
巻:
16
通号:
9
開始ページ:
817
終了ページ:
821
バージョン:
publisher
概要:
金沢大学理工研究域自然システム学系<br />A HPLC determination method for methamphetamine (MA) and its metabolites in the urine samples of abusers has been developed. MA, amphetamine (AP), norephedrine (NE), p-hydroxymethamphetamine (pOHMA), p-hydoroxya mphetamine (pOHAP) and an internal standard, namely β-phenylethylamine (PEA) were derivatized with dansyl chloride. They were separated on a reversed phase column with gradient elution using an acetonitrile/tetrahydrofuran/imidazole buffer mobile phase and chemilumigenically determined using bis(2, 4, 6-trichlorophenyl)-oxalate/hydrogen peroxide as post column reagents. The lower determination limits were as low as 1×10-14-3×10-14mol. AP, NE, pOHAP and PEA were derivatized with naphthalene-2, 3-dicarboxaldehyde, and were separated on a reversed phase column using an acetonitrile/imidazole buffer mobile phase and chemilumigenically determined. The lower determination limits were 3×10-16-1.5×10-15mol. Enzymatic hydrolysis of glucuronides of pOHMA (pOHMAG) and pOHAP (pOHAPG) allowed them to be determined as pOHMA and pOHAP, respectively. After adjusting the pH of the urine samples to 10.5 and adding PEA, all metabolites except glucuronides were extracted quantitatively into chloroform-isopropanol (3 : 1). Utilizing the two methods, MA and all metabolites were determined in urine samples of MA abusers. The tendency, in order of decreasing concentration was : [MA]>[A]>[pOHMAG]>[pOHMA]>[NE]>[pOHAPG]>[pOHAP]. Although ephedrine (EP) was detected in several samples, it was not considered to be a metabolite of MA but rather a component derived from cough medicine. 続きを見る
URL:
http://hdl.handle.net/2297/00063584
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