Visualization of Neural Activity in Insect Brains Using a Conserved Immediate Early Gene, Hr38

フォーマット:

論文

責任表示:

Fujita, Nozomi ; Nagata, Yuka ; Nishiuchi, Takumi ; Sato, Makoto ; Iwami, Masafumi ; Kiya, Taketoshi

言語:

英語

出版情報:

2013-10-21

著者名:

Fujita, Nozomi

Nagata, Yuka

Nishiuchi, Takumi

Sato, Makoto

Iwami, Masafumi

Kiya, Taketoshi

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掲載情報:

Current Biology

ISSN:

0960-9822      

巻:

23

通号:

20

開始ページ:

2063

終了ページ:

2070

バージョン:

author

概要:

Many insects exhibit stereotypic instinctive behavior [1-3], but the underlying neural mechanisms are not well understood due to difficulties in detecting brain activity in freely moving animals. Immediate early genes (IEGs), such as c-fos, whose expression is transiently and rapidly upregulated upon neural activity, are powerful tools for detecting behavior-related neural activity in vertebrates [4, 5]. In insects, however, this powerful approach has not been realized … because no conserved IEGs have been identified. Here, we identified Hr38 as a novel IEG that is transiently expressed in the male silkmoth Bombyx mori by female odor stimulation. Using Hr38 expression as an indicator of neural activity, we mapped comprehensive activity patterns of the silkmoth brain in response to female sex pheromones. We found that Hr38 can also be used as a neural activity marker in the fly Drosophila melanogaster. Using Hr38, we constructed a neural activity map of the fly brain that partially overlaps with fruitless (fru)-expressing neurons in response to female stimulation. These findings indicate that Hr38 is a novel and conserved insect neural activity marker gene that will be useful for a wide variety of neuroethologic studies. © 2013 Elsevier Ltd. All rights reserved. 続きを見る

URL:

http://hdl.handle.net/2297/36281