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Plasmids and their usefulness in molecular cloning |
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Bacteriophage λ and its vectors |
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Working with bacteriophage M13 vectors |
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Working with high-capacity vectors |
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Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis |
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Preparation and analysis of eukaryotic genomic DNA |
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Extraction, purification, and analysis of mRNA from eukaryotic cells |
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In vitro amplification of DNA by the polymerase chain reaction |
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Preparation of radiolabeled DNA and RNA probes |
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Working with synthetic oligonucleotide probes |
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Preparation of cDNA libraries and gene identification |
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DNA sequencing |
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Mutagenesis |
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Screening expression libraries |
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Expression of cloned genes in escherichia coli |
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Introducing cloned genes into cultured mammalian cells |
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Analysis of gene expression in mammalian cells |
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Protein interaction technologies |
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Plasmids and their usefulness in molecular cloning |
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Bacteriophage λ and its vectors |
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|
Working with bacteriophage M13 vectors |
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