※一部利用できない機能があります
| 1.
論文 |
論文
|
Maki, Teruya ; Aoki, Kazuma ; Kobayashi, Fumihisa ; Kakikawa, Makiko ; Tobo, Yutaka ; Matsuki, Atsushi ; Hasegawa, Hiroshi ; Iwasaka, Yasunobu
概要:
金沢大学理工研究域物質化学系<br />Microbial particles transported by Asian desert dust (KOSA) possibly impact ecosystems and human hea
…
lth in downwind environments and are commonly called "bioaerosols." The microbial communities associated with KOSA mineral particles (KOSA bioaerosol), which were collected from the snow cover on Mt. Tateyama, were investigated by means of a culture-amendment technique combined with denaturing gradient gel electrophoresis (DGGE) analysis using 16S rRNA genes. After the stratigraphy of the snow layer formed on the walls of a snow pit on Mt. Tateyama, samples were collected from 2 layers, which included KOSA particles and one which did not. The snow samples with KOSA particles indicated microbial growth in the 100 and 10-1 dilution media and in the medium with NaCl below 10%, while the snow sample without KOSA particles showed no microbial growth in the culture media. The PCR-DGGE analysis revealed that the bacterial compositions in the snow samples including KOSA mineral particles were mainly composed of the members of the phyla Actinobacteria, Firmicutus, and Proteobacteria. In particular, the 2 phylotypes appeared in the microbial cultures were similar to the members of the B. subtilis group, which has been detected in bioaerosol samples collected from the atmosphere over KOSA arrival (Suzu City) and source (Dunhuang City) regions. Presumably, halotolerant and oligotrophic bacterial communities are associated with the KOSA particles that descend to the snow cover on Mt. Tateyama. © 2011 Springer Science+Business Media B.V.
続きを見る
|
||||
| 2.
論文 |
論文
|
Puspitasari, Findya ; Maki, Teruya ; Shi, Guangyu ; Bin, Chen ; Kobayashi, Fumihisa ; Hasegawa, Hiroshi ; Iwasaka, Yasunobu
概要:
Airborne microorganisms (bioaerosol) from the China desert region, which are released into the atmosphere, disperse by t
…
he Asian dust event and affect ecosystems, human life, and atmospheric processes in downwind areas. However, the dynamics of airborne bacteria over the China desert regions have rarely been investigated. In this study, we analyzed bacterial communities in aerosols of the Asian dust source region (Taklimakan Desert) and compared them with the bacterial communities in sand dunes, for evaluating the mixtures from sand area to atmosphere. Air samples were collected at 10 m above the ground level from Dunhuang City during a dust event. The cell densities of airborne bacteria during a dust event were ten times more than that in non-dust periods. The 16S rDNA clone libraries from four air samples mainly belonged to two phyla, Firmicutes and Proteobacteria. During a dust event, the proportion of Proteobacteria clones decreased, whereas that of Firmicutes clones increased. Sand samples were collected from the sand dunes in four sampling sites of the Taklimakan Desert. The bacterial communities in sand samples comprised of the members of Firmicutes and Actinobacteria. The clones of Firmicutes in both air and sand samples included Bacillus species, constituting more than 10 % of total clones. Airborne bacterial communities would be carried by the dust events from sand dunes. Propionibacterium species from the class Actinobacteria that were dominant in sand samples were not detected in the air samples, suggesting that atmospheric stressors eliminate some bacterial species. Presumably, airborne bacterial communities in the Asian dust source region are composed of local environmental bacteria, and their dynamics depend on the occurrence of a dust event. © 2015 Springer Science+Business Media Dordrecht<br />Embargo Period 12 months
続きを見る
|
||||
| 3.
論文 |
論文
|
Kakikawa, Makiko ; Kobayashi, Fumihisa ; Maki, Teruya ; Yamada, Maromu ; Higashi, Tomomi ; Chen, Bin ; Shi, Guangyu ; Hong, Chunsang ; Tobo, Yutaka ; Iwasaka, Yasunobu
概要:
金沢大学環日本海域環境研究センター<br />The dust event injects microorganisms into the atmosphere and could facilitate the dispersal of b
…
iological particles affecting leeward ecosystem and human health. In this study, the dustborne microorganisms in the atmosphere over the Taklimakan Desert, Asian dust source, were identified by culture-independent method. Dusts were collect-ed using a balloon at about 800 m above the ground in an Asian dust source region, Dunhuang. After DNAwere directly extracted from the dusts collected filters, 16S and 18S rRNA genes of microorganisms were amplified, cloned, and sequenced. The rDNA sequence data indicated that dust particles include fungi closely related to Rickenella fibula, Ceriporiopsis gilvescens, and bacteria belonging to the genus Brevibacillus, Staphylococcus, Rhodococcus, Delftia, Pseudomonas, and Agrobacterium tumefaciens. These results suggest that dust particles in the atmosphere over Dunhuang could carry these many fungi and bacteria and might play a significant role in leeward ecosystem. © 2009 The Author(s).
続きを見る
|
||||
| 4.
論文 |
論文
|
Kakikawa, Makiko ; Kobayashi, Fumihisa ; Maki, Teruya ; Yamada, Maromu ; Higashi, Tomomi ; Chen, Bin ; Shi, Guangyu ; Hong, Chunsang ; Tobo, Yutaka ; Iwasaka, Yasunobu
概要:
金沢大学環日本海域環境研究センター生体機能計測研究部門<br />The dust event injects microorganisms into the atmosphere and could facilitate the disp
…
ersal of biological particles affecting leeward ecosystem and human health. In this study, the dustborne microorganisms in the atmosphere over the Taklimakan Desert, Asian dust source, were identified by culture-independent method. Dusts were collected using a balloon at about 800 m above the ground in an Asian dust source region, Dunhuang. After DNA were directly extracted from the dusts collected filters, 16S and 18S rRNA genes of microorganisms were amplified, cloned, and sequenced. The rDNA sequence data indicated that dust particles include fungi closely related to Rickenella fibula, Ceriporiopsis gilvescens, and bacteria belonging to the genus Brevibacillus, Staphylococcus, Rhodococcus, Delftia, Pseudomonas, and Agrobacterium tumefaciens. These results suggest that dust particles in the atmosphere over Dunhuang could carry these many fungi and bacteria and might play a significant role in leeward ecosystem. © 2008 Springer Science+Business Media B.V.
続きを見る
|
