CTEN/tensin 4 expression induces sensitivity to paclitaxel in prostate cancer

フォーマット:

論文

責任表示:

Li, You Qiang ; Mizokami, Atsushi ; Izumi, Kouji ; Narimoto, Kazutaka ; Shima, Takashi ; Zhang, Jian ; Dai, Jinlu ; Keller, Evan T. ; Namiki, Mikio

言語:

英語

出版情報:

Wiley-Blackwell, 2010-01-01

著者名:

Li, You Qiang

Mizokami, Atsushi

Izumi, Kouji

Narimoto, Kazutaka

Shima, Takashi

Zhang, Jian

Dai, Jinlu

Keller, Evan T.

Namiki, Mikio

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掲載情報:

Prostate

ISSN:

0270-4137      

巻:

70

通号:

1

開始ページ:

48

終了ページ:

60

バージョン:

author

概要:

金沢大学附属病院泌尿器科<br />BACKGROUND. Recently, we established paclitaxel-resistant prostate cancer cell lines (PC-3-TxR and DU145-TxR). To determine the mechanisms of paclitaxel resistance in PC-3-TxR cells, we compared the gene expression p … rofiles between PC-3 and PC-3-TxR cells. Our results indicated that expression of the C-terminal tensin like protein (CTEN, tensin 4) gene was down-regulated by 10-fold in PC-3-TxR cells. We investigated the possibility that CTEN overexpression restores paclitaxel sensitivity. METHODS. We investigated how knockdown and overexpression of CTEN in androgen-independent cell lines affect paclitaxel sensitivity by colony formation assay and growth inhibition assay. To determine the mechanisms by which CTEN affects paclitaxel sensitivity, we investigated the relationships between CTEN and F-actin or epidermal growth factor receptor (EGFR) in PC-3 cells. We also examined the association between expression of CTEN and grade of prostate cancer by immunohistochemistry using tissue microarray analysis. RESULTS. Down-regulation of CTEN, which is located in the cytoskeleton, played an important role in paclitaxel resistance in PC-3-TxR cells. Knockdown of CTEN expression in PC-3 cells induced paclitaxel resistance. Overexpression of CTEN in PC-3-TxR and DU145-TxR cells restored paclitaxel sensitivity. CTEN expression was inversely correlated with F-actin and EGFR expression. Then knockdown of actin and EGFR in PC-3-TxR cells recovered paclitaxel sensitivity, indicating that CTEN down-regulation mediates paclitaxel resistance through elevation of EGFR and actin expression. Moreover, CTEN expression was inversely correlated with Gleason score. CONCLUSIONS. These results strongly suggested that CTEN plays an important role in paclitaxel sensitivity and that CTEN expression level may be a prognostic predictive factor for PCa patients. © 2009 Wiley-Liss, Inc. 続きを見る

URL:

http://hdl.handle.net/2297/20335