Inhibition of epidermal growth factor-induced cell transformation and Akt activation by caffeine

フォーマット:

論文

責任表示:

Nomura, Masaaki ; Ichimatsu, Daisuke ; Moritani, Shuzo ; Koyama, Ichiko ; Dong, Zigang ; Yokogawa, Koichi ; Miyamoto, Kenichi

言語:

英語

出版情報:

Wiley-Liss, 2005-09-01

著者名:

Nomura, Masaaki

Ichimatsu, Daisuke

Moritani, Shuzo

Koyama, Ichiko

Dong, Zigang

Yokogawa, Koichi

Miyamoto, Kenichi

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掲載情報:

Molecular Carcinogenesis

ISSN:

0899-1987      

巻:

44

通号:

1

開始ページ:

67

終了ページ:

76

バージョン:

author

概要:

金沢大学医学部附属病院薬剤部<br />We found that caffeine significantly inhibited epidermal growth factor (EGF)- and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in the JB6 mouse epidermal cell line. The tumor promoter-indu … ced cell transformation was also blocked by treatment with an adenosine A1 receptor antagonist, 8-phenyltheophylline (8-PTH). Caffeine slightly attenuated activation of EGF-induced activator protein 1 (AP-1) activation, which play important roles in cell transformation, but only at the highest concentration examined (1 mM). Interestingly, pretreatment with caffeine suppressed EGF-induced phosphorylation and activation of Akt and ribosomal p70 S6 protein kinase (p70 S6K), a target of Akt, without inhibiting phosphatidylinositol 3-kinase (PI3K) activation. The inhibition of Akt activation of caffeine was not a result of its adenosine receptor antagonism. Because Akt plays a key role in signal transduction pathways leading to cell proliferation and apoptosis, our results provide novel insight into possible mechanisms of the chemotherapeutic effect of caffeine. © 2005 Wiley-Liss, Inc. 続きを見る

URL:

http://hdl.handle.net/2297/6720