Determination of Diamino- and Aminopyrenes by High Performance Liquid Chromatography with Chemiluminescence Detection

フォーマット:

論文

責任表示:

Hayakawa, Kazuichi ; Kitamura, Ryuichi ; Butoh, Mizuka ; Imaizumi, Noriko ; Miyazaki, Motoichi

言語:

英語

出版情報:

日本分析化学会 = The Japan Society for Analytical Chemistry, 1991-01-01

著者名:

• Hayakawa, Kazuichi
• Kitamura, Ryuichi
• Butoh, Mizuka
• Imaizumi, Noriko
• Miyazaki, Motoichi

掲載情報:

Analytical Sciences

ISSN:

0910-6340      

巻:

7

通号:

4

開始ページ:

573

終了ページ:

577

バージョン:

publisher

概要:

A high performance liquid chromatographic (HPLC) method with chemiluminescence (CL) detection was developed for the sensitive determination of 1, 3-diaminopyrene (1, 3-DAP), 1, 6-DAP, 1, 8-DAP and 1-aminopyrene (1-AP). The HPLC conditions were as follows: column, Cosmosil 5C18 (4.6mm i.d.×250mm); mobile phase, 10mM imidazole- perchloric acid buffer(pH 7.6)-acetonitrile (1:1, v/v); CL reagent, 0.02mM bis(2, 4, 6-trichlorophenyl)oxalate (TCPO) and 15mM … hydrogen peroxide in acetonitrile. The oxidative degradation of DAPS and AP in the presence of metals was prevented by adding ascorbic acid to sample solutions. The calibration curves were straight over 2 orders of magnitude for all analytes, and their detection limits (as S/N was 3) were in the sub-fmol range. Dinitro- and nitropyrenes in sooty emissions of diesel- and gasoline-engine cars could be determined by this HPLC after reductive conversion into DAPs and AP, respectively, by refluxing the samples in the presence of sodium hydrosulfide. 続きを見る

URL:

http://hdl.handle.net/2297/44556