Simple and cost-effective assay for isolating invasive living cells

フォーマット:

論文

責任表示:

Takino, Takahisa ; Sato, Hiroshi ; Seiki, Motoharu ; 滝野, 隆久

言語:

英語

出版情報:

Future Medicine, 2018-09

著者名:

• Takino, Takahisa
• Sato, Hiroshi
• Seiki, Motoharu
• 滝野, 隆久

掲載情報:

BioTechniques

ISSN:

0736-6205      

巻:

65

通号:

3

開始ページ:

137

終了ページ:

142

バージョン:

publisher

概要:

金沢大学国際基幹教育院GS教育系 / Institute of Liberal Arts and Science<br />A modified invasion assay using a three-dimensional collagen gel was developed that enables isolation of invasive living cells; it was named the invading cell trapping (iCT … ) assay. A small cell strainer consisting of a nylon mesh with 40-μm2 pores was used, and collagen gel layers formed across the membrane. Test cells were seeded in the lower gel layer and invasive cells were attracted upward and trapped in the upper gel. After incubation, the collagen gel layers in cell strainers were easily separated and living cells in the gel were counted and analyzed. An advantage of the iCT assay is that it can capture living invasive cells in the upper gel while leaving noninvasive ones in the lower layer. Further enrichment of the two cell populations can be achieved by repeating the assay. Thus, the iCT assay allows comparative analysis of invasive versus noninvasive cells. 続きを見る

URL:

http://hdl.handle.net/2297/00053869