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| 1.
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Hayakawa, Kazuichi ; Miyoshi, Yasuko ; Kurimoto, Hiroaki ; Matzushima, Yuko ; Takayama, Nariaki ; Tanaka, Seishi ; Miyazaki, Motoichi ; 早川, 和一 ; 三好, 裕子 ; 栗本, 博昭 ; 松島, 優子 ; 高山, 成明 ; 田中, 星司 ; 宮崎, 元一
概要:
金沢大学理工研究域自然システム学系<br />A HPLC determination method for methamphetamine (MA) and its metabolites in the urine samples of
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abusers has been developed. MA, amphetamine (AP), norephedrine (NE), p-hydroxymethamphetamine (pOHMA), p-hydoroxyamphetamine (pOHAP) and an internal standard, namely β-phenylethylamine (PEA) were derivatized with dansyl chloride. They were separated on a reversed phase column with gradient elution using an acetonitrile/tetrahydrofuran/imidazole buffer mobile phase and chemilumigenically determined using bis(2, 4, 6-trichlorophenyl)-oxalate/hydrogen peroxide as post column reagents. The lower determination limits were as low as 1×10-14-3×10-14mol. AP, NE, pOHAP and PEA were derivatized with naphthalene-2, 3-dicarboxaldehyde, and were separated on a reversed phase column using an acetonitrile/imidazole buffer mobile phase and chemilumigenically determined. The lower determination limits were 3×10-16-1.5×10-15mol. Enzymatic hydrolysis of glucuronides of pOHMA (pOHMAG) and pOHAP (pOHAPG) allowed them to be determined as pOHMA and pOHAP, respectively. After adjusting the pH of the urine samples to 10.5 and adding PEA, all metabolites except glucuronides were extracted quantitatively into chloroform-isopropanol (3 : 1). Utilizing the two methods, MA and all metabolites were determined in urine samples of MA abusers. The tendency, in order of decreasing concentration was : [MA]>[A]>[pOHMAG]>[pOHMA]>[NE]>[pOHAPG]>[pOHAP]. Although ephedrine (EP) was detected in several samples, it was not considered to be a metabolite of MA but rather a component derived from cough medicine.
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早川, 和一 ; 山本 , 敦 ; 宮崎, 元一 ; Hayakawa, Kazuichi ; Yamamoto, Atsushi ; Miyazaki, Motoichi
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早川, 和一 ; 宮崎, 元一 ; Hayakawa, Kazuichi ; Miyazaki, Motoichi
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今成, 登志男 ; 大久保, 登 ; 早川, 和一 ; 宮崎, 元一
概要:
生体試料中の金属を電気透析試料調製器によって陽イオンとして直接抽出する方法(A法)とEDTA-金属錯体として陰イオンとして抽出する方法(B法)について条件の検討を行った. A法の条件は泳動液0.05N酢酸,電流密度15mA/cm2,抽出時間
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(20～40)分,B法の条件は泳動液0.05N水酸化アンモニウム-1m M EDTA,電流密度20mA/cm2,抽出時問(20～40)分であった.A法では鉄(III),銅(II)の回収率80%,亜鉛(II)の回収率は90%,一方B法については鉄(III),亜鉛(II)の回収率100%,銅(II)の回収率は95%であった.たん白質として牛血清アルブミン,電解質として塩化ナトリウムの共存の影響を調べた後,B法を血しょう中のアルブミン結合亜鉛の分析に応用した. The extraction of metals from biological materials by an electrodialytic sample preparator was studied. Two procedures are proposed in this method : one depends on the extraction of metals as cationic ions (Mode A) and the other depends on the extraction of metal-EDTA complexes as anionic ions (Mode B). The basic conditions for Mode A are : carrier solution; 0.05 N CH3COOH, current density; 15 mA/cm2, extraction time; (2040) min. The conditions for Mode B are : carrier solution; 0.05 N NH4OH-1 mM EDTA, current density; 20 mA/cm2 and extraction time; (2040)min. The recoveries of Fe(III), Cu(II) and Zn(II) in Mode A were 90, 80 and 90%, and in Mode B the recoveries were 100, 95 and 100% respectively. The effects of electrolytes and proteins on the extraction of metals were examined, and the application of Mode B for the determination of albumin bound zinc in human plasma was carried out as follows : the supernatant containing albumin bound zinc was obtained by polyethylene glycol treatment and subjected to electrodialysis for 40 min according to the conditions described in Mode B. Albumin bound zinc in the extract was determined by atomic absorption spectrometry (AAS). Total plasma zinc was measured according to direct dilution method by AAS and α2-macroglobulin bound zinc was obtained by the difference.
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山本, 敦 ; 松永, 明信 ; 関口, 久義 ; 早川, 和一 ; 宮崎, 元一
概要:
Photometric ion chromatography is a novel method by using the photometer as a detector. A feature of this method is the use of light absorbing ion in the mobil phase, and the appearance of transparent sample ion in the effluent is
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detected by "trough"in the base line absorbance. We applied this technique by using a conventional HPLC system equipped with a commercially available ion exchange separation column to the determination of bromate in such foodstuffs as bread and kamaboko. Sample solution was prepared by aqueous extraction and mixed with acetone for removal of proteins. After evaporation of the organic solvent, the aqueous solution was dechlorinated by a DOWEX column (Ag-form), and then was subjected to chromatography. Samples of bread and kamaboko were spiked with 5.4 ppm and 8.4 ppm of bromate, respectively, and examined by the above method. The average recoveries were 88.3% in bread and 82.9% in kamaboko, and the coefficients of variation were 7.7% and 7.9%, respectively. The detection limit was 1 ppm. The above findings indicate that bromate can be detected by HPLC using conventional instrumentation, and the sensitivity of this system is comparable to conductimetric detection.
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早川, 和一 ; 山本, 敦 ; 宮崎, 元一
概要:
Ion chromatography (IC), which was introduced in 1975, has become widely used for the sensitive and simultaneous determination of ions. In the early stage, the IC system consisted of two ion-exchange columns (separator and suppressor,
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respectively) and a conductivity detector. Although this combination is still the most popular, new forms of separation and suppression have been developed. On the other hand, an indirect photometric detection using a UV detector has become popular in IC and other detectors commonly used in HPLC also have been used. Each of them has specific advantages for different types of analyses. Thus, IC is now defined as a form of liquid chromatography of ions. This article reviews new developments of the different forms of IC and their applications, especially to environmental samples, foods, biological materials and drugs, from reports published since 1990.
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吉田, 育世 ; 早川, 和一 ; 宮崎, 元一
概要:
Simultaneous determination of carboxylic acids and inorganic anions was investigated by photometric ion chromatography w
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ith phthalate solution as an eluent. The retention time and the peak area of carboxylic acids changed remarkably with eluent pH. The optimum conditions at present were as follows : analytical column, OYOBUNKO ASA-4000 (4.6 mm i.d.×25 cm) ; eluent, 5×10-4 M disodium phthalate ; flow rate, 2 ml/min ; column temperature, 40°C ; detection wavelength, 240 nm ; injection volume, 100 μl. The detection limits for several carboxylic acids as 3 times noise were 0.05-0.07 mg/l in sample solution. Their calibration curves showed straight lines by the peak area method. The present method has an advantage to determine simultaneously not only carboxylic acids but also inorganic anions. Under the condition described above, both carboxylic acids and inorganic anions in several liquid foods were easily determined by direct injection of the sample prepared with only dilution.
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松永, 明信 ; 山本, 敦 ; 水上, 英一 ; 早川, 和一 ; 宮崎, 元一
概要:
A high-performance liquid chromatographic method using post column reaction system was established for the determination
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of polyphosphates (PP). Linear polyphosphates with the range of polymerization from 2 to 9 (P2-P9) could be separated on an anion exchange column, TSK GEL DEAE-5PW (Toyo Soda), with 0.01 M HNO3 containing NaNO3 as a mobile phase at flow rate of 1.0 ml/min, with a linear gradient in NaNO3 concentration at one hour intervals. PP were detected as the decrease of absorbance at 500 nm of ferric-sulfosalicylate complex by means of an on-line post column reaction at room temperature, with 0.5 mM FeCl3 containing 2.5 mM sulfosalicylic acid as reaction reagent at flow rate of 0.5 ml/min. The proposed method was applied to the analysis of 30 different commercial products of soft drinks. The pretreatment only necessary after mixing the sample with an equal volume of 0.01 M HNO3 was filtration through 0.45 μm pore size filter. P2-P6 (total 0.231 g/kg) and P2-P4 (0.070 g/kg) were found from 2 kinds of the samples, respectively. The determination limit was 0.02 m mol/kg for all of the PP.
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宮崎, 元一 ; 早川, 和一 ; 飯田, 寿恵 ; 藤井, ちず子
概要:
A simple method by using a column packed with Amberlyst-15 resin was established to remove metal ions which affect on PO
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V and TBA tests for lipid peroxidation. A methanol solution (2 ml) of lipid containing metal ions was applied to the column (0.5 cm×5 cm), and eluted with methanol (6 ml). Metal ions such as Cu2+, Fe3+ and Mn2+ were retained on the column, whereas linoleic acid, linolenic acid and methyl linolenate were completely recovered in the eluate (8 ml). By testing the peroxidized lipid with POV and TBA methods, the recoveries of the lipid mixture after the procedure were more than 85% (POV) and more than 99% (TBA). This pretreatment by using Amberlyst-15 column is available for the peroxidation tests of lipid.
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山本, 敦 ; 松永, 明信 ; 牧野, 正雄 ; 早川, 和一 ; 宮崎, 元一
概要:
A method for the simultaneous determination of organic acids was investigated by photometric ion chromatography by using
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two different eluents. A 4.6 mm i.d.×25 cm stainless column packed with MCI GEL SCA 01 (Mitsubishi Chemical Ind., Ltd.) was used as a separator column. Sample was injected into this column previously equilibrated with 1 mM citraconic acid at pH 6.8. After elution of monobasic organic acids, the eluent was exchanged to a stronger one, 1 mM m-sulfobenzoic acid at pH 6.8. Dibasic organic acids were eluted before the column reequilibration with the secondary eluent. On the other hand, tribasic organic acids such as citric acid were eluted with the secondary eluent. To prevent the base line disorder due to the eluent exchange, the determination at 254 nm was performed. Because this wave length is an isosbestic point of the two eluents. Sour-taste agents in soft drinks could be analysed directly after dilution of samples.
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早川, 和一 ; 宮崎, 元一
概要:
Ion chromatography (IC) with conductivity detection, which was introduced in 1975, has become widely used for the simultaneous determination of inorganic and organic ions. On the other hand, photometric ion chromatography (PIC) based on
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the difference of absorbances of sample and eluent (mobile phase) species has attracted much attention in the recent field of IC, since this technique has potential advantages over IC methods with conductivity detection. This technique which allows IC on a conventional single column HPLC system with an absorbance detector gives compatible sensitivity to conductivity detection. This paper deals mainly with indirect PIC methods performed by both ion exchange and reversedphase modes, where non-UV-absorbing ions are detected as positive and negative peaks by using UV-absorbing ions in the eluent (mobile phase). The features of PIC have been reasonably well known, but it is necessary to understand principles and conditions of PIC for the effective use of this technique. This paper reviews a number of significant reports which deal with the optimisations of separating column, eluent (mobile phase), detection wavelength, sensitivity and restrictions of PIC with its applications. It is also the purpose of this paper to discuss alternative methods and innovations of PIC.
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早川, 和一 ; 西尾, 和佳子 ; 宮崎, 元一
概要:
The lipoperoxidation in plasma was the most accelerated by the addition of 8-hydroxyquinoline (8HQ)-Fe (III) chelate among all 8HQ-metal chelate systems examined. The same tendency was also observed for 5-chloro-7-iodo-8HQ (C)-Fe
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(III) chelate system. The activity of 8HQ-Fe (III) system in lipoperoxidation was the highest at [8HQ]:[Fe (III)]=1: 1. In quinoline derivatives, the ligands which have 8-hydroxyl group and high solubility in lipid were necessary for lipoperoxidation in plasma by Fe (III) chelate systems. From above facts, both of the denaturation of nervous systems in SMON patients ad ministered with C and the antibiotic effects of 8HQ may be considered as the results of lipoperoxidation by C-Fe (III) chelate and 8HQ-Fe (III) chelate, respectively.
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| 13.
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木津, 良一 ; 東, 信一 ; 早川, 和一 ; 宮崎, 元一
概要:
金沢大学医薬保健研究域薬学系<br />血しょう及び尿中の制がん性白金錯体オキザラート(トランス-l-1,2-ジアミノシクロヘキサン)プラチニウム(II)(l-OHP)の分析法を研究した.種々検討した結果,分析条件は以下のように定めた.前処
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理;Dowex 1-X8,Dowex 50 W-X4及びSep-Pak C_<18>カートリッジを一連のカラムとして構成する.この前処理カラムに血しょう及び尿の1mlと引き続き水を流す.前処理カラム溶出液の最初の6ml部分は捨て,次の10ml部分を分取し,高速液体クロマトグラフィー(HPLC)の試料とした.HPLC条件;カラム:Finepak SIL C_<18>(4.6mm i.d.×25cm),カラム温度:50℃,溶離液:水-メタノール(95:5),流速:1ml/min,検出波長:210nm,試料注入量:100μlピーク高さによる検量線は,原点を通る良い直線性を示した.定量下限は,血しょう及び尿中l-OHP濃度0.5μM,再現性はR.S.D.3%程度であった.血しょう及び尿中のl-OHPを高感度で精度良く定量することができた. A method for determination of (trans-l-1, 2-diaminocyclohexane) oxalatoplatinum (II) (l-OHP), introduced by Kidani et al. as one of the promising antineoplastic platinum complexes, in plasma and urine by high performance liquid chromatography (HPLC) was investigated in order to contribute for metabolic study of l-OHP. The recommended pretreatment procedures and HPLC conditions are as follows. Pretreatment of plasma and urine ; pretreatment column is composed of Dowex 1-X8 (5mm i. d.×6cm), Dowex 50W-X4 (5 mm i. d.×3 cm) and Sep-Pak C_<18> cartridge. Blood samples are centrifuged at 1000 g for 3 min to obtain plasma. One ml of plasma or urine is immediately placed on the pretreatment column and is eluted with water at the flow rate of 1.2 ml/min. The initial 6 ml portion of effluent is discarded and subsequent 10 ml portion is collected. An aliquot (100 μl) of the effluent collected is subjected to HPLC. All of these pretreatment procedures are carried out at 5±2℃. HPLC conditions ; column : Finepak SIL C_<18> (4.6 mm i. d.×25cm), eluent : H_2O-MeOH (95 : 5), column temperature : 40℃, flow rate : 1 ml/min, detection wavelength : 210 nm, injection volume : 100 μl. Under the conditions described above, a linear relationship was obtained between peak height and l-OHP concentration in plasma and urine. Determination limit was 0.5 μM l-OHP in sample solutions. Recovery of l-OHP in pretreatment was 86±3% in plasma and 83±2% in urine. A sensitive and precise determination of l-OHP in plasma and urine will be possible by this method.
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早川, 和一 ; 蛯名, 令子 ; 松本, 美枝子 ; 宮崎, 元一
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金沢大学医薬保健研究域薬学系<br />Determination of inorganic anions and cations in some vegetable juices was examined by ultraviolet p
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hotometric ion chromatography with a conventional HPLC system equipped with a UV monitor and a separating column packed with popular ion exchangers. Inorganic anions such as Cl^-, NO_3^-, and SO_4^<2-> in sample solution were completely separated on a Zipax SAX packed column (50 cm × 2.1 mm I. D.) with 5 × 10^<-3> M disodium phthalate as an eluent and detected at 240 nm. Inorganic cations such as Na^+, NH_4^+, K^+, Mg^<2+>, and Ca^<3+> in sample solution were completely separated on a Zipax SCX packed column (25 cm × 4.6 mm I. D.) with 2.5 × 10^<-3> M copper sulfate as an eluent and detected at 220 nm. Under the conditions described above, the three anions in juices of Chinese cabbage, tomato, and radish, and the five cations in Sep-pak C_<18> treated juice of Chinese cabbage were determined.
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綿谷, 敏彦 ; 早川, 和一 ; 能村, 京子 ; 宮崎, 元一
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金沢大学医薬保健研究域薬学系<br />The differential chromatogram method was useful to reduce large changes in background response in si
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ngle column ion chromatography using stepwise elution. Cations (Na^+, K^+, Mg^<2+> and Ca^<2+>) were separated on cation exchange column by stepwise elution using both 0.01 mM and 3.0 mM copper sulfate eluents and detected at 230 nm by indirect photometry. Anions (lactate, acetate, propionate, formate, citrate, Cl^-, NO_2^-, Br^-, NO_3^-, SO_4^<2->, S_2O_3^<2-> and I^-) were separated on an anion exchange column using both 0.3 mM and 1.5 mM disodium phthalate eluents and detected at 270 nm. This method was also successfully applied to conductometric detection.
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石川, 雅章 ; 山本, 政利 ; 増井, 俊夫 ; 早川, 和一 ; 宮崎, 元一 ; 中澤, 裕之 ; 藤田, 昌彦
概要:
金沢大学医薬保健研究域薬学系<br />Determination of inorganic ions in aqueous infusions was studied by photometric ion chromatography (
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PIC) using a conventional HPLC system equipped with a UV monitor. Inorganic cations (Na^+, K^+, Mg^<2+> and Ca^<2+>) could be separated on a strong cation exchange column, Oyobunko ASC-4000 column (4.6 mm i. d.×250 mm), with 1×10^<-4> M CuSO_4 for Na^+, K^+ and 2×10^<-3> M CuSO_4 for Mg^<2+>, Ca^<2+> as eluents, and detected at 220 nm. Inorganic anions (Cl^-, PO_4^<3-> and SO_4^<2->) could be separated on a strong anion exchange column, Oyobunko ASA-4000 column (4.6 mm i. d.×250 mm), with 1×10^<-3> M disodium phthal- ate (pH 10) as an eluent, and detected at 260 nm. A sample solution was simply diluted with distilled water, and could be analyzed by PIC. Relative standard deviations (n=5) for above ions in a commercial infusion were below 2.0%.
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村橋, 毅 ; 早川, 和一 ; 岩本, 侑子 ; 宮崎, 元一
概要:
金沢大学医薬保健研究域薬学系<br />A simultaneous determination method for polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs (NPAH
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s) in airborne particulates was developed. Airborne particulates were extracted with benzene/ethanol, cleaned up by liquid-liquid partition, refluxed in the presence of NaSH and then the resultant solution was injected into an HPLC system. The system was hybridized into two systems with different optimum conditions for PAHs and NPAHs by introducing a switching valve and a mini separation column. Mobile phases were acetonitrile/water (75 : 25) for PAHs, and acetonitrile/imidazole HClO_4 buffer (1 : 1) for NPAHs and the postcolumn reagent solution for NPAHs was an acetonitrile solution containing bis(2,4,6-trichlorophenyl)oxalate and H_2O_2. By this method, four NPAHs (1,3-, 1,6-, 1,8-dinitropyrenes and 1-nitropyrene) and eight PAHs (fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene and coronene) in sub-milligram quantities of airborne particulates were determined chemilumigenically and fluorogenically, respectively using 2-fluoro-7-nitrofluorene as an internal standard within sixty minutes.
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中村, 誠治 ; 今泉, 範子 ; 早川, 和一 ; 宮崎, 元一
概要:
金沢大学医薬保健研究域薬学系<br />ノンサプレッサー型イオンクロマトグラフィー(IC)としてはん用される電気伝導度検出ICと間接吸光度検出ICの感度特性を検出原理式に基づき,強酸性物質と弱酸性物質について比較した.両法に用いることができ
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る溶離液種として安息香酸及びフタル酸を用いると,硝酸イオンなどの強酸性物質の検出では,低pH溶離液の電気伝導度検出のほうが感度が高く,弱酸性物質の検出では,高pH溶離液の間接吸光度検出のほうが高かった.共通溶離液としてはん用される低pHのフタル酸溶離液を用いたときの両者による強酸性物質の分析値の相関は良かった.又,溶離液として,1,5-ナフタレンジスルホン酸やベンゼンジスルホン酸を用いると,間接吸光度検出による感度を向上させることができ,強酸性物質の検出でも低pHフタル酸溶離液を用いたときの感度を上回った. The detection sensitivities of anionic compounds by conductivity detection (CD) and indirect photometric detection (IPD) were compared in non-suppressed ion chromatography. The reciprocal of peak area to noise ratio was used to compare the two detection methods for such common eluents as benzoate and phthalate at pH 4.3 and pH 6.7. The sensitivities of strong acid compounds were highest by CD at pH 4.3, while those of weak acid compounds were highest by IPD at pH 6.7. These results were consistent with theoretical equations for the two detection methods. The sensitivities of strong acid compounds by IPD exceeded those by CD with the phthalate eluent at pH 4.3, using eluent species such as 1, 5-naphthalenedisulfonate. Correlations between the two methods were excellent in the determination of inorganic anions in soluble fractions of monthly collected deposits
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早川, 和一 ; 平木, 博美 ; 宮崎, 元一
概要:
金沢大学医薬保健研究域薬学系<br />入手が容易なイオン交換カラムを備えた普及型HPLC装置で可能な,無機陰イオンの微量分析条件を検討した.無機陰イオン(炭酸イオン,塩化物イオン,亜硝酸イオン,リン酸イオン,臭化物イオン,硝酸イオン,硫酸
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イオン,ヨウ化物イオン)は,フタル酸二ナトリウム溶液を溶離液として,陰イオン交換カラムで分離溶出された後,ベースラインの谷として紫外検出される.炭酸イオン,塩化物イオン,リン酸イオン,臭化物イオン,硝酸イオン,硫酸イオンの同時分析の最適条件は,カラム:Zipax SAX (2.1 mm i.d.×50cm):溶離液:5×10^<-4> Mフクル酸二ナトリウム;流速:1 ml/min; 温度:室温;検出波長:240 nm;注入量:100μlであった.この条件で,検出下限はppbレベルであり,ピーク高さ法と面積法のいずれでも検量線は直線となった.本法を用いると,環境試料中の主要無機陰イオンが短時間に一斉分析でき,しかも本法の分析値は,他法の結果ともよく一致した. A photometric determination method of microamounts of inorganic anions has been developed by conventional high performance liquid chromatography using a popular ion exchange column. Inorganic anions (HCO_3^-, Cl^-, NO_2^-, H_2PO_4^-, Br^-, NO_3^-, SO_4^<2->, and I^-) were separated on an anoin exchange column by using disodium phthalate solution as an eluent, and detected as troughs in the base line absorbance by a UV monitor. The optimum conditions for the determination of HCO_3^-, C1^-, H_2PO_4^-, Br^-, NO_3^-, and SO_4^<2-> were as follows : column, Zipax SAX (2.1 mm i.d. ×50cm) ; eluent, 5×10^<-4>M disodium phthalate ; flow rate, 1 ml/min ; column temperature, ambient ; detection wavelength, 240 nm. Under the conditions described above, detection limits of these anions were at levels as low as ppb. The calibration curves gave straight lines by either peak height or peak area method. Main inorganic anions contained in environmental samples could be simultaneously determined by the proposed method, and their analytical results were close to those obtained by other methods.
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早川, 和一 ; 平木, 博美 ; 宮崎, 元一
概要:
金沢大学医薬保健研究域薬学系<br />A novel method for the determination of micro amounts of inorganic ions has been developed. The sepa
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ration of alkaline metal ions (Li^+, Na^+, and K^+) was accomplished on a Zipax SCX packed column (4.6 mm × 12 cm) with 2.5×10^<-4> M copper sulfate as an eluent, and the separation of halogen ions (F^-, Cl^-, and Br^-) was accomplished on a Zipax SAX packed column (4.6 mm × 15cm) with 1.0 × 10^<-3> M sodium benzoate as an eluent. The eluted ions were detected as troughs in base line absorbance of eluent ion at 220 nm or 240 nm. Under the conditions described above, the six ions were easily separated and determined at the levels as low as ppb by using either a peak height method or a peak area method which gave straight calibration curves. Other inorganic ions can be also determined by changing composition and concentration of the eluent. Moreover, this analytical method is economical and simple, since it can be performed with a conventional HPLC system equipped with a single separation column packed with inexpensive and easily available ion exchanger and a photometric detector.
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論文 |
論文
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早川, 和一 ; 西島, 美知子 ; 宮崎, 元一
概要:
金沢大学医薬保健研究域薬学系<br />A novel method for the separation of halogenated anion complexes of platinum metals by reversed-phas
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e ion-pair partition chromatography has been developed. The separation of RhBr_5^<2->, PdBr_4^<2->,and PtBr_6^<2-> was accomplished on a Hitachi #3050 packed column (3mm×30cm), where 30 % (v/v) acetonitrile-0.02 M phosphate buffer (pH 7) mixture containing 0.1 M tetrabutylammonium bromide and 0.025 M sodium bromide was used as a mobile phase. These anion complexes can be determined at 310nm, and as low as 5×10^<-11> mol of PtBr_6^<-2> can be determined with the peak height method which gave a straight calibration curve. Detecting wavelengths and retention times of the complexes can be altered if the complexes are formed with other halogen ions. This analytical method for micro amounts of platinum metals can remove the mutual metal interferences.
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