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骨制御分子Runx2による中枢制御機構の解明

フォーマット:
論文
責任表示:
宝田, 剛志 ; Takarada, Takeshi
言語:
日本語
出版情報:
金沢大学医薬保健研究域薬学系, 2013-05-23
著者名:
掲載情報:
平成24(2012)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 = 2012 Fiscal Year Final Research Report
巻:
2010-2012
開始ページ:
4p.
バージョン:
author
概要:
金沢大学医薬保健研究域薬学系<br />我々は、骨芽細胞特異的表現型を特徴付けるRunt-related transcription factor 2(Runx2)分子が、中枢神経系にも機能的に発現する事実を報告した。本研究では、個体レベルでのRunx2の脳機能解析を実施する目的で、Cre/loxPシステムを利用したRunx2 conditional欠損マウスの作製に取り組み、Runx2遺伝子のexon 4をloxP配列にて挟んだRunx2^flox/+のマウス作 製に成功した。<br />We have previously shown the functional expression of glutamatergic and GABAergic signaling machineries in different osseous cells including osteoblasts. Runt-related factor 2 (Runx2) is the master regulator of osteoblastic differentiation with ability to accelerate differentiation of mesenchymal stem cells into osteoblasts, while we have also demonstrated the expression of mRNA and corresponding protein for Runx2. In this study, we for the first time generated mice carrying a conditional Runx2 allele with exon 4, which encodes the Runt domain, flanked by loxP sites. These mice were crossed with α1(I)-collagen-Cre or α1(II)-collagen-Cre transgenic mice to obtain osteoblast- or chondrocyte-specific Runx2 deficient mice, respectively. In newborn α1(II)-Cre;Runx2^flox/flox mice, mineralization impairment was restricted to skeletal areas undergoing endochondral ossification including long bones and vertebrae. In contrast, no apparent skeletal abnormalities were seen in mutant embryo, newborn, and 3- to 6-week old-mice in which Runx2 had been deleted with the α1(I)-collagen-Cre driver. The Runx2 floxed allele established here is undoubtedly useful for investigating the role of Runx2 in particular cells.<br />研究課題/領域番号:22500330, 研究期間(年度):2010-2012 続きを見る
URL:
http://hdl.handle.net/2297/00050080
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宝田, 剛志

日本薬学会 = Pharmaceutical Society of Japan

宝田, 剛志, Takarada, Takeshi

金沢大学医薬保健研究域薬学系

杉山, 和久, Sugiyama, Kazuhisa

金沢大学医薬保健研究域医学系