Blank Cover Image

The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells

フォーマット:
論文
責任表示:
Aung, Khin Thuzar ; Yoshioka, Kazuaki ; Aki, Sho ; Ishimaru, Kazuhiro ; Takuwa, Noriko ; Takuwa, Yoh ; 吉岡, 和晃 ; 安藝, 翔 ; 多久和, 典子 ; 多久和, 陽
言語:
英語
出版情報:
Physiological Society of Japan 日本生理学会 / Springer Nature, 2019-03-01
著者名:
Aung, Khin Thuzar
Yoshioka, Kazuaki
Aki, Sho
Ishimaru, Kazuhiro
Takuwa, Noriko
Takuwa, Yoh
吉岡, 和晃
安藝, 翔
多久和, 典子
多久和, 陽
続きを見る
掲載情報:
Journal of Physiological Sciences
ISSN:
1880-6546  CiNii Articles  Webcat Plus  JAIRO
巻:
69
通号:
2
開始ページ:
263
終了ページ:
280
バージョン:
author
概要:
金沢大学医薬保健研究域医学系<br />Pinocytosis is an important fundamental cellular process that is used by the cell to transport fluid and solutes. Phosphoinositide 3-kinases (PI3Ks) regulate a diverse array of dynamic membrane events. However, it is not well-understood which PI3K isoforms are involved in specific mechanisms of pinocytosis. We performed knockdown studies of endogenous PI3K isoforms and clathrin heavy chain (CHC) mediated by small interfering RNA (siRNA). The results demonstrated that the class II PI3K PI3K-C2α and PI3K-C2β, but not the class I or III PI3K, were required for pinocytosis, based on an evaluation of fluorescein-5-isothiocyanate (FITC)–dextran uptake in endothelial cells. Pinocytosis was partially dependent on both clathrin and dynamin, and both PI3K-C2α and PI3K-C2β were required for clathrin-mediated—but not clathrin-non-mediated—FITC-dextran uptake at the step leading up to its delivery to early endosomes. Both PI3K-C2α and PI3K-C2β were co-localized with clathrin-coated pits and vesicles. However, PI3K-C2β, but not PI3K-C2α, was highly co-localized with actin filament-associated clathrin-coated structures and required for actin filament formation at the clathrin-coated structures. These results indicate that PI3K-C2α and PI3K-C2β play differential, indispensable roles in clathrin-mediated pinocytosis. © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature.<br />Embargo Period 12 months 続きを見る
URL:
http://hdl.handle.net/2297/00053883
タイトル・著者・出版者が同じ資料

類似資料:

1
 
2
 
3
 
4
 
5
 
6
 
7
 
8
 
9
 
10
 
11
 
12
 

Pham, Hoa Q., Yoshioka, Kazuaki, Mohri, Hiromi, Nakata, Hiroki, Aki, Sho, Ishimaru, Kazuhiro, Takuwa, Noriko, Takuwa, &hellip;

John Wiley & Sons

Aki, Sho, Yoshioka, Kazuaki, Okamoto, Yasuo, Takuwa, Noriko, Takuwa, Yoh

American Society for Biochemistry and Molecular Biology

Sarker, Md Azadul Kabir, Aki, Sho, Yoshioka, Kazuaki, Kuno, Kouji, Okamoto, Yasuo, Ishimaru, Kazuhiro, Takuwa, Noriko, &hellip;

Endocrine Society / Oxford University Press

Zhao, Juanjuan, Okamoto, Yasuo, Asano, Yuya, Ishimaru, Kazuhiro, Aki, Sho, Yoshioka, Kazuaki, Takuwa, Noriko, Wada, &hellip;

Public Library of Science

多久和, 陽, Takuwa, Yoh

金沢大学医薬保健研究域医学系

小泉, 早織, 吉岡, 和晃, 安藝, 翔, 多久和, 陽

金沢大学十全医学会 = The Juzen Medical Society Kanazawa University

吉岡, 和晃, 多久和, 陽

金沢大学十全医学会 = The Juzen Medical Society Kanazawa University

宮澤, 秀和, 吉岡, 和晃, 多久和, 陽

金沢大学十全医学会