1.

図書

図書
based on the motion picture story by James Schamus ; screenplay by John Turman and Michael France and James Schamus ; retold by David Maule
出版情報: Harlow : Pearson Education, 2004
シリーズ名: Penguin readers ; Level 2
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2.

図書

図書
Francis Celoria
出版情報: London ; New York : Routledge, 1992
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3.

論文

論文
Roy, Anuradha ; Shimizu, Sakiko ; Kiya, Taketoshi ; Mita, Kazuei ; Iwami, Masafumi
出版情報: Zoological Science.  29  pp.333-339,  2012-05-01.  日本動物学会
URL: http://hdl.handle.net/2297/31376
概要: The insect brain secretes prothoracicotropic hormone (PTTH), which stimulates the prothoracic gland to synthesize ecdyso ne. The active metabolite of ecdysone, 20-hydroxyecdysone (20E), works through ecdysone receptor (EcR) and ultraspiracle (USP) to initiate molting and metamorphosis by regulating downstream genes. Previously, we found that EcR was expressed in the PTTH-producing neurosecretory cells (PTPCs) in larval brain of the silkworm Bombyx mori, suggesting that PTPCs function as the master cells of development under the regulation of 20E. To gain a better understanding of the molecular mechanism of the 20E control of PTPCs, we performed a comprehensive screening of genes induced by 20E using DNA microarray with brains of day-2 fifth instar silkworm larvae. Forty-one genes showed greater than twofold changes caused by artificial application of 20E. A subsequent semiquantitative screening identified ten genes upregulated by 20E, four of which were novel or not previously identified as 20E-response genes. Developmental profiling determined that two genes, UP4 and UP5, were correlated with the endogenous ecdysteroid titer. Whole-mount in situ hybridization showed exclusive expression of these two genes in two pairs of cells in the larval brain in response to 20E-induction, suggesting that the cells are PTPCs. BLAST searches revealed that UP4 and UP5 are Bombyx homologs of vrille and tarsal-less, respectively. The present study identifies 20E-induced genes that may be involved in the ecdysone signal hierarchies underlying pupal-adult development and/or the 20E regulation of PTPCs. © 2012 Zoological Society of Japan.<br />発行後1年より全文公開 続きを見る
4.

論文

論文
Urata, Makoto ; Yamaguchi, Masaaki
出版情報: Zoological science.  21  pp.533-540,  2004-05-01.  Zoological Society of Japan = 日本動物学会
URL: http://hdl.handle.net/2297/28996
概要: We describe development from fertilization to metamorphosis of the enteropneust hemichordate Balanoglossus misakiensis. This is the first report to describe the complete development of an indirect-developing hemichordate under laboratory conditions. Mature adults were induced to spawn by shifting the temperature of seawater from 23 to 28°C. Eggs (200 μm diameter) were enclosed within a non-mucilaginous membrane, and dispersed readily in seawater. After artificial insemination, a fertilization envelope was elevated from the egg surface beneath the egg membrane; this was followed by the formation of the first and second polar bodies within the envelope. Zygotes cleaved at 20-min intervals to form blastulae, and gastrulation started 9 h after fertilization. Embryos hatched 1 day after fertilization to become typical feeding tornaria larvae. The larvae metamorphosed 7-10 days after fertilization without undergoing the first (Müller) or forth (Krohn) stage of indirect-developing hemichordate development. Larvae that were not fed failed to metamorphose. Juveniles completed adult body formation within a week of settling in sand at the bottom of the culture tube. We discuss heterochronical modifications of B. misakiensis development, and make the case for this species as a potential model organism for the investigation of indirect-developing hemichordates. 続きを見る
5.

論文

論文
Suzuki, Takumi ; Sakurai, Sho ; Iwami, Masafumi
出版情報: Pesticide Biochemistry and Physiology.  97  pp.199-203,  2010-07-01.  Elsevier
URL: http://hdl.handle.net/2297/23495
概要: 金沢大学理工研究域自然システム学系<br />Holometabolous insects develop without feeding and excreting during the pupal period and thus req uire repository organs for metabolic waste, or meconium. The rectal sac is an organ for storing meconium during pupal-adult development of holometabolous insects. Although the rectal sac has an essential function, hormonal and developmental regulation of waste-accumulation and the consequences of rectal sac distention are still unknown. In the silkworm, Bombyx mori, the rectal sac distends with meconium in the middle pupal period under the regulation of ecdysteroid. Here, we show that juvenile hormone analog (JHA) delayed rectal sac distention and disturbed adult emergence. Distention was not restored completely by an injection of 20-hydroxyecdysone (20E) into pupae applied with JHA, suggesting that JHA suppresses 20E action and delays the timing of ecdysteroid elevation. Thus the "status quo" action of JHA may function in two different ways during pupal-adult development. © 2010 Elsevier Inc. All rights reserved. 続きを見る
6.

論文

論文
Suzuki, Takumi ; Sakurai, Sho ; Iwami, Masafumi
出版情報: Journal of Insect Physiology.  56  pp.673-677,  2010-06-01.  Elsevier
URL: http://hdl.handle.net/2297/25021
概要: 金沢大学理工研究域自然システム学系<br />Successful insect development is achieved via appropriate fluctuation of ecdysteroid levels. When an insect's ecdysteroid level is disrupted, physiological and developmental defects occur. In the pupa of the silkworm, Bombyx mori, the rectal sac is an essential organ that operates as a repository for degraded ecdysteroids, and it can be distended by administration of 20-hydroxyecdysone (20E). Our previous study showed that rectal sac distention appears 4 days after 20E administration. Hemolymph ecdysteroid levels, however, decrease to lower level during this period. Thus, the timing of the rectal sac distention does not match with that of ecdysteroid elevation. Here, we examine how 20E induces rectal sac distention. A ligature experiment and ecdysteroid quantification showed that continuous 20E stimulation induces rectal sac distention. Thorax tissue contributed to the continuous 20E stimulation needed to induce distention. Ecdysteroid released from the thorax tissue may be converted to 20E by ecdysone 20-hydroxylase to produce continuous 20E stimulation. Thus, the ecdysone metabolic pathway plays a critical role in rectal sac distention. © 2010 Elsevier Ltd. 続きを見る
7.

論文

論文
Suzuki, Takumi ; Sakurai, Sho ; Iwami, Masafumi
出版情報: Journal of Insect Physiology.  55  pp.250-254,  2009-03-01.  Elsevier
URL: http://hdl.handle.net/2297/14386
概要: Holometabolous insects do not excrete but store metabolic wastes during the pupal period. The waste is called meconium a nd is purged after adult emergence. Although the contents of meconium are well-studied, the developmental and physiological regulation of meconium accumulation is poorly understood. In Bombyx mori, meconium is accumulated in the rectal sac; thereby, the rectal sac distends at the late pupal stage. Here, we show that rectal sac distention occurs between 4 and 5 days after pupation. The distention is halted by brain-removal just after larval-pupal ecdysis but not by brain-removal 1 day after pupation. In the pupae, brain-removal just after ecdysis kept the hemolymph ecdysteroid titer low during early and mid-pupal stages. An injection of 20-hydroxyecdysone (20E) evoked the distention that was halted by brain-removal in a dose-dependent manner. Therefore, brain-removal caused the lack of ecdysteroid, and rectal sac distention did not appear in the brain-removed pupae because of the lack of ecdysteroid. We conclude that rectal sac distention is one of the developmental events regulated by 20E during the pupal period in B. mori. © 2008 Elsevier Ltd. All rights reserved. 続きを見る
8.

論文

論文
Suzuki, Takumi ; Sakurai, Sho ; Iwami, Masafumi
出版情報: Pesticide Biochemistry and Physiology.  97  pp.199-203,  2010-07-01.  Elsevier
URL: http://hdl.handle.net/2297/24744
概要: 金沢大学理工研究域自然システム学系<br />Holometabolous insects develop without feeding and excreting during the pupal period and thus req uire repository organs for metabolic waste, or meconium. The rectal sac is an organ for storing meconium during pupal-adult development of holometabolous insects. Although the rectal sac has an essential function, hormonal and developmental regulation of waste-accumulation and the consequences of rectal sac distention are still unknown. In the silkworm, Bombyx mori, the rectal sac distends with meconium in the middle pupal period under the regulation of ecdysteroid. Here, we show that juvenile hormone analog (JHA) delayed rectal sac distention and disturbed adult emergence. Distention was not restored completely by an injection of 20-hydroxyecdysone (20E) into pupae applied with JHA, suggesting that JHA suppresses 20E action and delays the timing of ecdysteroid elevation. Thus the "status quo" action of JHA may function in two different ways during pupal-adult development. © 2010 Elsevier Inc. 続きを見る
9.

論文

論文
Suzuki, Takumi ; Sakurai, Sho ; Iwami, Masafumi
出版情報: Journal of Insect Physiology.  56  pp.673-677,  2010-06-01.  Elsevier
URL: http://hdl.handle.net/2297/23874
概要: 金沢大学理工研究域自然システム学系<br />Successful insect development is achieved via appropriate fluctuation of ecdysteroid levels. When an insect's ecdysteroid level is disrupted, physiological and developmental defects occur. In the pupa of the silkworm, Bombyx mori, the rectal sac is an essential organ that operates as a repository for degraded ecdysteroids, and it can be distended by administration of 20-hydroxyecdysone (20E). Our previous study showed that rectal sac distention appears 4 days after 20E administration. Hemolymph ecdysteroid levels, however, decrease to lower level during this period. Thus, the timing of the rectal sac distention does not match with that of ecdysteroid elevation. Here, we examine how 20E induces rectal sac distention. A ligature experiment and ecdysteroid quantification showed that continuous 20E stimulation induces rectal sac distention. Thorax tissue contributed to the continuous 20E stimulation needed to induce distention. Ecdysteroid released from the thorax tissue may be converted to 20E by ecdysone 20-hydroxylase to produce continuous 20E stimulation. Thus, the ecdysone metabolic pathway plays a critical role in rectal sac distention. © 2010 Elsevier Ltd. All rights reserved. 続きを見る
10.

論文

論文
Monwar, Hossain ; Shimizu, Sakiko ; Matsuki, Masahiko ; Imamura, Masanori ; Sakurai, Sho ; Iwami, Masafumi
出版情報: Insect Biochemistry and Molecular Biology.  38  pp.1001-1007,  2008-11-01.  Elsevier
URL: http://hdl.handle.net/2297/12347
概要: 金沢大学理工研究域自然システム学系<br />The insect brain is the center of developmental control, from which ecdysone governs brain morpho genesis and regulates gene expression cascades associated with molting and metamorphosis. In order to identify the 20-hydroxyecdysone (20E)-inducible genes responsible for molting and metamorphosis, we constructed a 20E-induced subtraction complementary DNA library from the fifth instar larval brain of the silkworm Bombyx mori. We isolated 10 genes, designated as bombeil-1 to bombeil-10, three of which did not show any sequence similarity to previously identified Bombyx genes. Whole-mount in situ hybridization revealed that all of these bombeil messenger RNAs were exclusively located in two pairs of lateral neurosecretory cells in the larval brain, known as prothoracicotropic hormone (PTTH)-producing cells. RNA-interference knockdown targeting bombeil-2 resulted in larval-pupal molt defects, and adult wing and leg malformations. These results, together with the cell-specific co-localization of bombeil transcripts with PTTH, suggest that bombeil genes play important roles during larval-pupal-adult development. © 2008 Elsevier Ltd. All rights reserved 続きを見る